image lab software package Search Results


densitometry analysis  (Bio-Rad)
99
Bio-Rad densitometry analysis
DC-SIGN engagement induces autophagy and associates with LC3. ( A ) HEK293T cells were co-transfected with plasmids expressing DC-SIGN WT and GST-expressing constructs as indicated. Cells were engaged or not with DC-SIGN mAbs (5 μg/mL) for 30 min before pull-down. Lysates (input control) and GST pull-down were immunoblotted with anti-DC-SIGN ( upper panels), anti-GST ( middle panels), and anti-GAPDH for input control. This experiment is representative of 4. ( B ) Lysates from MoDC treated for indicated times with DC-SIGN specific mAbs were immunoblotted with anti-LC3, and loading was controlled with anti-GAPDH ( upper blots). Negative control experiments were performed using lysates of cells incubated with non-specific mouse serum (Isotype Ab) and immunoblotted as before ( lower blots). Pre-treatments of MoDC with chloroquine (50 μM) were performed to validate functional autophagy flux as evidenced by LC3-II increase. <t>Densitometry</t> values and LC3-II/GAPDH ratio obtained from lysates of primary MoDC treated with DC-SIGN mAbs (n = 6) or Isotype mouse Abs (n = 3) were obtained for each time point of each condition and graphically reported on the right. The same experiment as above was performed but with lysates from MoDC treated with ( C ) ManLAM (2 μg/mL) (n = 4) or ( D ) challenged with HIV-1-R5 (MOI of 2) (n = 3) for indicated times. LC3-II/GAPDH ratio from densitometry analyses was graphically represented as before. Statistical significance: * = p < 0.05; ** = p < 0.01.
Densitometry Analysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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densitometry analysis - by Bioz Stars, 2026-04
99/100 stars
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image labtm software version 6 0 1  (Bio-Rad)
99
Bio-Rad image labtm software version 6 0 1
DC-SIGN engagement induces autophagy and associates with LC3. ( A ) HEK293T cells were co-transfected with plasmids expressing DC-SIGN WT and GST-expressing constructs as indicated. Cells were engaged or not with DC-SIGN mAbs (5 μg/mL) for 30 min before pull-down. Lysates (input control) and GST pull-down were immunoblotted with anti-DC-SIGN ( upper panels), anti-GST ( middle panels), and anti-GAPDH for input control. This experiment is representative of 4. ( B ) Lysates from MoDC treated for indicated times with DC-SIGN specific mAbs were immunoblotted with anti-LC3, and loading was controlled with anti-GAPDH ( upper blots). Negative control experiments were performed using lysates of cells incubated with non-specific mouse serum (Isotype Ab) and immunoblotted as before ( lower blots). Pre-treatments of MoDC with chloroquine (50 μM) were performed to validate functional autophagy flux as evidenced by LC3-II increase. <t>Densitometry</t> values and LC3-II/GAPDH ratio obtained from lysates of primary MoDC treated with DC-SIGN mAbs (n = 6) or Isotype mouse Abs (n = 3) were obtained for each time point of each condition and graphically reported on the right. The same experiment as above was performed but with lysates from MoDC treated with ( C ) ManLAM (2 μg/mL) (n = 4) or ( D ) challenged with HIV-1-R5 (MOI of 2) (n = 3) for indicated times. LC3-II/GAPDH ratio from densitometry analyses was graphically represented as before. Statistical significance: * = p < 0.05; ** = p < 0.01.
Image Labtm Software Version 6 0 1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/image labtm software version 6 0 1/product/Bio-Rad
Average 99 stars, based on 1 article reviews
image labtm software version 6 0 1 - by Bioz Stars, 2026-04
99/100 stars
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imagelab software version 5 2 1  (Bio-Rad)
99
Bio-Rad imagelab software version 5 2 1
DC-SIGN engagement induces autophagy and associates with LC3. ( A ) HEK293T cells were co-transfected with plasmids expressing DC-SIGN WT and GST-expressing constructs as indicated. Cells were engaged or not with DC-SIGN mAbs (5 μg/mL) for 30 min before pull-down. Lysates (input control) and GST pull-down were immunoblotted with anti-DC-SIGN ( upper panels), anti-GST ( middle panels), and anti-GAPDH for input control. This experiment is representative of 4. ( B ) Lysates from MoDC treated for indicated times with DC-SIGN specific mAbs were immunoblotted with anti-LC3, and loading was controlled with anti-GAPDH ( upper blots). Negative control experiments were performed using lysates of cells incubated with non-specific mouse serum (Isotype Ab) and immunoblotted as before ( lower blots). Pre-treatments of MoDC with chloroquine (50 μM) were performed to validate functional autophagy flux as evidenced by LC3-II increase. <t>Densitometry</t> values and LC3-II/GAPDH ratio obtained from lysates of primary MoDC treated with DC-SIGN mAbs (n = 6) or Isotype mouse Abs (n = 3) were obtained for each time point of each condition and graphically reported on the right. The same experiment as above was performed but with lysates from MoDC treated with ( C ) ManLAM (2 μg/mL) (n = 4) or ( D ) challenged with HIV-1-R5 (MOI of 2) (n = 3) for indicated times. LC3-II/GAPDH ratio from densitometry analyses was graphically represented as before. Statistical significance: * = p < 0.05; ** = p < 0.01.
Imagelab Software Version 5 2 1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/imagelab software version 5 2 1/product/Bio-Rad
Average 99 stars, based on 1 article reviews
imagelab software version 5 2 1 - by Bioz Stars, 2026-04
99/100 stars
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image lab version 6 0 1 software  (Bio-Rad)
99
Bio-Rad image lab version 6 0 1 software
DC-SIGN engagement induces autophagy and associates with LC3. ( A ) HEK293T cells were co-transfected with plasmids expressing DC-SIGN WT and GST-expressing constructs as indicated. Cells were engaged or not with DC-SIGN mAbs (5 μg/mL) for 30 min before pull-down. Lysates (input control) and GST pull-down were immunoblotted with anti-DC-SIGN ( upper panels), anti-GST ( middle panels), and anti-GAPDH for input control. This experiment is representative of 4. ( B ) Lysates from MoDC treated for indicated times with DC-SIGN specific mAbs were immunoblotted with anti-LC3, and loading was controlled with anti-GAPDH ( upper blots). Negative control experiments were performed using lysates of cells incubated with non-specific mouse serum (Isotype Ab) and immunoblotted as before ( lower blots). Pre-treatments of MoDC with chloroquine (50 μM) were performed to validate functional autophagy flux as evidenced by LC3-II increase. <t>Densitometry</t> values and LC3-II/GAPDH ratio obtained from lysates of primary MoDC treated with DC-SIGN mAbs (n = 6) or Isotype mouse Abs (n = 3) were obtained for each time point of each condition and graphically reported on the right. The same experiment as above was performed but with lysates from MoDC treated with ( C ) ManLAM (2 μg/mL) (n = 4) or ( D ) challenged with HIV-1-R5 (MOI of 2) (n = 3) for indicated times. LC3-II/GAPDH ratio from densitometry analyses was graphically represented as before. Statistical significance: * = p < 0.05; ** = p < 0.01.
Image Lab Version 6 0 1 Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/image lab version 6 0 1 software/product/Bio-Rad
Average 99 stars, based on 1 article reviews
image lab version 6 0 1 software - by Bioz Stars, 2026-04
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bio rad imagelab touch 2 4 software  (Bio-Rad)
99
Bio-Rad bio rad imagelab touch 2 4 software
DC-SIGN engagement induces autophagy and associates with LC3. ( A ) HEK293T cells were co-transfected with plasmids expressing DC-SIGN WT and GST-expressing constructs as indicated. Cells were engaged or not with DC-SIGN mAbs (5 μg/mL) for 30 min before pull-down. Lysates (input control) and GST pull-down were immunoblotted with anti-DC-SIGN ( upper panels), anti-GST ( middle panels), and anti-GAPDH for input control. This experiment is representative of 4. ( B ) Lysates from MoDC treated for indicated times with DC-SIGN specific mAbs were immunoblotted with anti-LC3, and loading was controlled with anti-GAPDH ( upper blots). Negative control experiments were performed using lysates of cells incubated with non-specific mouse serum (Isotype Ab) and immunoblotted as before ( lower blots). Pre-treatments of MoDC with chloroquine (50 μM) were performed to validate functional autophagy flux as evidenced by LC3-II increase. <t>Densitometry</t> values and LC3-II/GAPDH ratio obtained from lysates of primary MoDC treated with DC-SIGN mAbs (n = 6) or Isotype mouse Abs (n = 3) were obtained for each time point of each condition and graphically reported on the right. The same experiment as above was performed but with lysates from MoDC treated with ( C ) ManLAM (2 μg/mL) (n = 4) or ( D ) challenged with HIV-1-R5 (MOI of 2) (n = 3) for indicated times. LC3-II/GAPDH ratio from densitometry analyses was graphically represented as before. Statistical significance: * = p < 0.05; ** = p < 0.01.
Bio Rad Imagelab Touch 2 4 Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bio rad imagelab touch 2 4 software/product/Bio-Rad
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bio rad imagelab touch 2 4 software - by Bioz Stars, 2026-04
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chemidoc touch  (Bio-Rad)
99
Bio-Rad chemidoc touch
DC-SIGN engagement induces autophagy and associates with LC3. ( A ) HEK293T cells were co-transfected with plasmids expressing DC-SIGN WT and GST-expressing constructs as indicated. Cells were engaged or not with DC-SIGN mAbs (5 μg/mL) for 30 min before pull-down. Lysates (input control) and GST pull-down were immunoblotted with anti-DC-SIGN ( upper panels), anti-GST ( middle panels), and anti-GAPDH for input control. This experiment is representative of 4. ( B ) Lysates from MoDC treated for indicated times with DC-SIGN specific mAbs were immunoblotted with anti-LC3, and loading was controlled with anti-GAPDH ( upper blots). Negative control experiments were performed using lysates of cells incubated with non-specific mouse serum (Isotype Ab) and immunoblotted as before ( lower blots). Pre-treatments of MoDC with chloroquine (50 μM) were performed to validate functional autophagy flux as evidenced by LC3-II increase. <t>Densitometry</t> values and LC3-II/GAPDH ratio obtained from lysates of primary MoDC treated with DC-SIGN mAbs (n = 6) or Isotype mouse Abs (n = 3) were obtained for each time point of each condition and graphically reported on the right. The same experiment as above was performed but with lysates from MoDC treated with ( C ) ManLAM (2 μg/mL) (n = 4) or ( D ) challenged with HIV-1-R5 (MOI of 2) (n = 3) for indicated times. LC3-II/GAPDH ratio from densitometry analyses was graphically represented as before. Statistical significance: * = p < 0.05; ** = p < 0.01.
Chemidoc Touch, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chemidoc touch - by Bioz Stars, 2026-04
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imaging processing toolkit mevislab  (MeVIS Research GmbH)
90
MeVIS Research GmbH imaging processing toolkit mevislab
DC-SIGN engagement induces autophagy and associates with LC3. ( A ) HEK293T cells were co-transfected with plasmids expressing DC-SIGN WT and GST-expressing constructs as indicated. Cells were engaged or not with DC-SIGN mAbs (5 μg/mL) for 30 min before pull-down. Lysates (input control) and GST pull-down were immunoblotted with anti-DC-SIGN ( upper panels), anti-GST ( middle panels), and anti-GAPDH for input control. This experiment is representative of 4. ( B ) Lysates from MoDC treated for indicated times with DC-SIGN specific mAbs were immunoblotted with anti-LC3, and loading was controlled with anti-GAPDH ( upper blots). Negative control experiments were performed using lysates of cells incubated with non-specific mouse serum (Isotype Ab) and immunoblotted as before ( lower blots). Pre-treatments of MoDC with chloroquine (50 μM) were performed to validate functional autophagy flux as evidenced by LC3-II increase. <t>Densitometry</t> values and LC3-II/GAPDH ratio obtained from lysates of primary MoDC treated with DC-SIGN mAbs (n = 6) or Isotype mouse Abs (n = 3) were obtained for each time point of each condition and graphically reported on the right. The same experiment as above was performed but with lysates from MoDC treated with ( C ) ManLAM (2 μg/mL) (n = 4) or ( D ) challenged with HIV-1-R5 (MOI of 2) (n = 3) for indicated times. LC3-II/GAPDH ratio from densitometry analyses was graphically represented as before. Statistical significance: * = p < 0.05; ** = p < 0.01.
Imaging Processing Toolkit Mevislab, supplied by MeVIS Research GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imaging processing toolkit mevislab - by Bioz Stars, 2026-04
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radiology imaging software package  (Impax Inc)
90
Impax Inc radiology imaging software package
DC-SIGN engagement induces autophagy and associates with LC3. ( A ) HEK293T cells were co-transfected with plasmids expressing DC-SIGN WT and GST-expressing constructs as indicated. Cells were engaged or not with DC-SIGN mAbs (5 μg/mL) for 30 min before pull-down. Lysates (input control) and GST pull-down were immunoblotted with anti-DC-SIGN ( upper panels), anti-GST ( middle panels), and anti-GAPDH for input control. This experiment is representative of 4. ( B ) Lysates from MoDC treated for indicated times with DC-SIGN specific mAbs were immunoblotted with anti-LC3, and loading was controlled with anti-GAPDH ( upper blots). Negative control experiments were performed using lysates of cells incubated with non-specific mouse serum (Isotype Ab) and immunoblotted as before ( lower blots). Pre-treatments of MoDC with chloroquine (50 μM) were performed to validate functional autophagy flux as evidenced by LC3-II increase. <t>Densitometry</t> values and LC3-II/GAPDH ratio obtained from lysates of primary MoDC treated with DC-SIGN mAbs (n = 6) or Isotype mouse Abs (n = 3) were obtained for each time point of each condition and graphically reported on the right. The same experiment as above was performed but with lysates from MoDC treated with ( C ) ManLAM (2 μg/mL) (n = 4) or ( D ) challenged with HIV-1-R5 (MOI of 2) (n = 3) for indicated times. LC3-II/GAPDH ratio from densitometry analyses was graphically represented as before. Statistical significance: * = p < 0.05; ** = p < 0.01.
Radiology Imaging Software Package, supplied by Impax Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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radiology imaging software package - by Bioz Stars, 2026-04
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image-pro plus 7.0  (Evident Corporation)
90
Evident Corporation image-pro plus 7.0
DC-SIGN engagement induces autophagy and associates with LC3. ( A ) HEK293T cells were co-transfected with plasmids expressing DC-SIGN WT and GST-expressing constructs as indicated. Cells were engaged or not with DC-SIGN mAbs (5 μg/mL) for 30 min before pull-down. Lysates (input control) and GST pull-down were immunoblotted with anti-DC-SIGN ( upper panels), anti-GST ( middle panels), and anti-GAPDH for input control. This experiment is representative of 4. ( B ) Lysates from MoDC treated for indicated times with DC-SIGN specific mAbs were immunoblotted with anti-LC3, and loading was controlled with anti-GAPDH ( upper blots). Negative control experiments were performed using lysates of cells incubated with non-specific mouse serum (Isotype Ab) and immunoblotted as before ( lower blots). Pre-treatments of MoDC with chloroquine (50 μM) were performed to validate functional autophagy flux as evidenced by LC3-II increase. <t>Densitometry</t> values and LC3-II/GAPDH ratio obtained from lysates of primary MoDC treated with DC-SIGN mAbs (n = 6) or Isotype mouse Abs (n = 3) were obtained for each time point of each condition and graphically reported on the right. The same experiment as above was performed but with lysates from MoDC treated with ( C ) ManLAM (2 μg/mL) (n = 4) or ( D ) challenged with HIV-1-R5 (MOI of 2) (n = 3) for indicated times. LC3-II/GAPDH ratio from densitometry analyses was graphically represented as before. Statistical significance: * = p < 0.05; ** = p < 0.01.
Image Pro Plus 7.0, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vessel diameter software inturis cardio image viewing package release 1.2  (Philips Healthcare)
90
Philips Healthcare vessel diameter software inturis cardio image viewing package release 1.2
DC-SIGN engagement induces autophagy and associates with LC3. ( A ) HEK293T cells were co-transfected with plasmids expressing DC-SIGN WT and GST-expressing constructs as indicated. Cells were engaged or not with DC-SIGN mAbs (5 μg/mL) for 30 min before pull-down. Lysates (input control) and GST pull-down were immunoblotted with anti-DC-SIGN ( upper panels), anti-GST ( middle panels), and anti-GAPDH for input control. This experiment is representative of 4. ( B ) Lysates from MoDC treated for indicated times with DC-SIGN specific mAbs were immunoblotted with anti-LC3, and loading was controlled with anti-GAPDH ( upper blots). Negative control experiments were performed using lysates of cells incubated with non-specific mouse serum (Isotype Ab) and immunoblotted as before ( lower blots). Pre-treatments of MoDC with chloroquine (50 μM) were performed to validate functional autophagy flux as evidenced by LC3-II increase. <t>Densitometry</t> values and LC3-II/GAPDH ratio obtained from lysates of primary MoDC treated with DC-SIGN mAbs (n = 6) or Isotype mouse Abs (n = 3) were obtained for each time point of each condition and graphically reported on the right. The same experiment as above was performed but with lysates from MoDC treated with ( C ) ManLAM (2 μg/mL) (n = 4) or ( D ) challenged with HIV-1-R5 (MOI of 2) (n = 3) for indicated times. LC3-II/GAPDH ratio from densitometry analyses was graphically represented as before. Statistical significance: * = p < 0.05; ** = p < 0.01.
Vessel Diameter Software Inturis Cardio Image Viewing Package Release 1.2, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vessel diameter software inturis cardio image viewing package release 1.2/product/Philips Healthcare
Average 90 stars, based on 1 article reviews
vessel diameter software inturis cardio image viewing package release 1.2 - by Bioz Stars, 2026-04
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particle image analysis software package 4.7  (Clemex Technologies Inc)
90
Clemex Technologies Inc particle image analysis software package 4.7
DC-SIGN engagement induces autophagy and associates with LC3. ( A ) HEK293T cells were co-transfected with plasmids expressing DC-SIGN WT and GST-expressing constructs as indicated. Cells were engaged or not with DC-SIGN mAbs (5 μg/mL) for 30 min before pull-down. Lysates (input control) and GST pull-down were immunoblotted with anti-DC-SIGN ( upper panels), anti-GST ( middle panels), and anti-GAPDH for input control. This experiment is representative of 4. ( B ) Lysates from MoDC treated for indicated times with DC-SIGN specific mAbs were immunoblotted with anti-LC3, and loading was controlled with anti-GAPDH ( upper blots). Negative control experiments were performed using lysates of cells incubated with non-specific mouse serum (Isotype Ab) and immunoblotted as before ( lower blots). Pre-treatments of MoDC with chloroquine (50 μM) were performed to validate functional autophagy flux as evidenced by LC3-II increase. <t>Densitometry</t> values and LC3-II/GAPDH ratio obtained from lysates of primary MoDC treated with DC-SIGN mAbs (n = 6) or Isotype mouse Abs (n = 3) were obtained for each time point of each condition and graphically reported on the right. The same experiment as above was performed but with lysates from MoDC treated with ( C ) ManLAM (2 μg/mL) (n = 4) or ( D ) challenged with HIV-1-R5 (MOI of 2) (n = 3) for indicated times. LC3-II/GAPDH ratio from densitometry analyses was graphically represented as before. Statistical significance: * = p < 0.05; ** = p < 0.01.
Particle Image Analysis Software Package 4.7, supplied by Clemex Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/particle image analysis software package 4.7/product/Clemex Technologies Inc
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particle image analysis software package 4.7 - by Bioz Stars, 2026-04
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tomtec research-arena software  (Tomtec Inc)
90
Tomtec Inc tomtec research-arena software
DC-SIGN engagement induces autophagy and associates with LC3. ( A ) HEK293T cells were co-transfected with plasmids expressing DC-SIGN WT and GST-expressing constructs as indicated. Cells were engaged or not with DC-SIGN mAbs (5 μg/mL) for 30 min before pull-down. Lysates (input control) and GST pull-down were immunoblotted with anti-DC-SIGN ( upper panels), anti-GST ( middle panels), and anti-GAPDH for input control. This experiment is representative of 4. ( B ) Lysates from MoDC treated for indicated times with DC-SIGN specific mAbs were immunoblotted with anti-LC3, and loading was controlled with anti-GAPDH ( upper blots). Negative control experiments were performed using lysates of cells incubated with non-specific mouse serum (Isotype Ab) and immunoblotted as before ( lower blots). Pre-treatments of MoDC with chloroquine (50 μM) were performed to validate functional autophagy flux as evidenced by LC3-II increase. <t>Densitometry</t> values and LC3-II/GAPDH ratio obtained from lysates of primary MoDC treated with DC-SIGN mAbs (n = 6) or Isotype mouse Abs (n = 3) were obtained for each time point of each condition and graphically reported on the right. The same experiment as above was performed but with lysates from MoDC treated with ( C ) ManLAM (2 μg/mL) (n = 4) or ( D ) challenged with HIV-1-R5 (MOI of 2) (n = 3) for indicated times. LC3-II/GAPDH ratio from densitometry analyses was graphically represented as before. Statistical significance: * = p < 0.05; ** = p < 0.01.
Tomtec Research Arena Software, supplied by Tomtec Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


DC-SIGN engagement induces autophagy and associates with LC3. ( A ) HEK293T cells were co-transfected with plasmids expressing DC-SIGN WT and GST-expressing constructs as indicated. Cells were engaged or not with DC-SIGN mAbs (5 μg/mL) for 30 min before pull-down. Lysates (input control) and GST pull-down were immunoblotted with anti-DC-SIGN ( upper panels), anti-GST ( middle panels), and anti-GAPDH for input control. This experiment is representative of 4. ( B ) Lysates from MoDC treated for indicated times with DC-SIGN specific mAbs were immunoblotted with anti-LC3, and loading was controlled with anti-GAPDH ( upper blots). Negative control experiments were performed using lysates of cells incubated with non-specific mouse serum (Isotype Ab) and immunoblotted as before ( lower blots). Pre-treatments of MoDC with chloroquine (50 μM) were performed to validate functional autophagy flux as evidenced by LC3-II increase. Densitometry values and LC3-II/GAPDH ratio obtained from lysates of primary MoDC treated with DC-SIGN mAbs (n = 6) or Isotype mouse Abs (n = 3) were obtained for each time point of each condition and graphically reported on the right. The same experiment as above was performed but with lysates from MoDC treated with ( C ) ManLAM (2 μg/mL) (n = 4) or ( D ) challenged with HIV-1-R5 (MOI of 2) (n = 3) for indicated times. LC3-II/GAPDH ratio from densitometry analyses was graphically represented as before. Statistical significance: * = p < 0.05; ** = p < 0.01.

Journal: International Journal of Molecular Sciences

Article Title: The Autophagy Nucleation Factor ATG9 Forms Nanoclusters with the HIV-1 Receptor DC-SIGN and Regulates Early Antiviral Autophagy in Human Dendritic Cells

doi: 10.3390/ijms24109008

Figure Lengend Snippet: DC-SIGN engagement induces autophagy and associates with LC3. ( A ) HEK293T cells were co-transfected with plasmids expressing DC-SIGN WT and GST-expressing constructs as indicated. Cells were engaged or not with DC-SIGN mAbs (5 μg/mL) for 30 min before pull-down. Lysates (input control) and GST pull-down were immunoblotted with anti-DC-SIGN ( upper panels), anti-GST ( middle panels), and anti-GAPDH for input control. This experiment is representative of 4. ( B ) Lysates from MoDC treated for indicated times with DC-SIGN specific mAbs were immunoblotted with anti-LC3, and loading was controlled with anti-GAPDH ( upper blots). Negative control experiments were performed using lysates of cells incubated with non-specific mouse serum (Isotype Ab) and immunoblotted as before ( lower blots). Pre-treatments of MoDC with chloroquine (50 μM) were performed to validate functional autophagy flux as evidenced by LC3-II increase. Densitometry values and LC3-II/GAPDH ratio obtained from lysates of primary MoDC treated with DC-SIGN mAbs (n = 6) or Isotype mouse Abs (n = 3) were obtained for each time point of each condition and graphically reported on the right. The same experiment as above was performed but with lysates from MoDC treated with ( C ) ManLAM (2 μg/mL) (n = 4) or ( D ) challenged with HIV-1-R5 (MOI of 2) (n = 3) for indicated times. LC3-II/GAPDH ratio from densitometry analyses was graphically represented as before. Statistical significance: * = p < 0.05; ** = p < 0.01.

Article Snippet: Chemiluminescence was acquired (Chemidoc, Bio-Rad Laboratories, Hercules, CA, USA), followed by densitometry analysis (Image Lab TM software; Bio-Rad Laboratories, Hercules, CA, USA).

Techniques: Transfection, Expressing, Construct, Control, Negative Control, Incubation, Functional Assay

ATG9 is required for DC-SIGN-mediated autophagy flux activation. Primary MoDC were treated with irrelevant siRNA (siCtrl) or siRNA against ATG9 (siATG9), and ATG9 expression was controlled by RT-qPCR ( left graph). Lysates from MoDC transfected as above and pre-treated with bafilomycin A1 (50 nM) for 1 h before stimulation with ManLAM (2 μg/mL) for 2 h were immunoblotted with anti-LC3 ( upper blot). The loading control was performed with anti-actin ( lower blot). LC3-II/actin ratio from densitometry analyses obtained from 3 independent experiments (n = 3) was normalized to untreated controls of each siRNA condition and graphically represented ( right graph). Statistical significance: * = p < 0.05.

Journal: International Journal of Molecular Sciences

Article Title: The Autophagy Nucleation Factor ATG9 Forms Nanoclusters with the HIV-1 Receptor DC-SIGN and Regulates Early Antiviral Autophagy in Human Dendritic Cells

doi: 10.3390/ijms24109008

Figure Lengend Snippet: ATG9 is required for DC-SIGN-mediated autophagy flux activation. Primary MoDC were treated with irrelevant siRNA (siCtrl) or siRNA against ATG9 (siATG9), and ATG9 expression was controlled by RT-qPCR ( left graph). Lysates from MoDC transfected as above and pre-treated with bafilomycin A1 (50 nM) for 1 h before stimulation with ManLAM (2 μg/mL) for 2 h were immunoblotted with anti-LC3 ( upper blot). The loading control was performed with anti-actin ( lower blot). LC3-II/actin ratio from densitometry analyses obtained from 3 independent experiments (n = 3) was normalized to untreated controls of each siRNA condition and graphically represented ( right graph). Statistical significance: * = p < 0.05.

Article Snippet: Chemiluminescence was acquired (Chemidoc, Bio-Rad Laboratories, Hercules, CA, USA), followed by densitometry analysis (Image Lab TM software; Bio-Rad Laboratories, Hercules, CA, USA).

Techniques: Activation Assay, Expressing, Quantitative RT-PCR, Transfection, Control